极小种群植物“川柿”生物学特性及种子萌发方法研究

摘 要：【目的】为了保护和发展极小种群植物川柿，开展此研究。【方法】采用观测法，连续3年对其生物学特性研究；种子萌发方法：（1）后熟0、30、45、60天，剥离清洗播种；（2）后熟60天，以GA300、600、1000 mg/L溶液浸种1h；播种基质为河沙（保持一定湿度），放置室内，统计萌发时间和出苗率。【结果】（1）川柿花期4-5月，10月为果熟期，单果直径32.7±5.80mm，单果重23.0±5.30g；种子肾形、千粒重1070g；成年植株呈隔年开花、结果现象；（2）种子经“后熟+河沙播种”，可萌发，其中以后熟60天的出苗率较高，为65%；直接播种种子，出苗率5%；“后熟+GA溶液浸种+河沙播种”，出苗率均高于65%，其中以GA600mg/L浸泡1h处理，出苗率最高为83.3%。【结论】极小种群植物川柿为常绿叶乔木，有隔年开花、结果现象； “果后熟60天+GA600mg/L浸泡种子1h +室内河沙播种”可提高种子萌发率，为川柿种子繁殖提供参考。     

Sacred sites as habitats of culturally important plant species in an Indian megacity

Cultural ecosystem services related to urban green spaces contribute significantly to liveable cities. While previous studies highlight the intersection of cultural ecosystem services with societal values, spiritual or religious values associated with urban nature have received less attention. In India, as in other parts of the world, sacred sites are known for their biological richness, but analyses from urban sacred sites are largely missing. Based on a stratified random sampling approach, we analysed the cultivated and wild plant species assemblages of 69 sacred sites in the megacity of Bengaluru, India, in relation to biological and cultural features, and parameters related to the urban matrix and type of sacred sites (temple vs. katte). Unlike other urban studies, we found a dominance of native species in the cultivated and spontaneous species pools (121 species in total), with Ficus religiosa and Azadirachta indica as most frequently planted species. Culturally relevant species prevailed in the species pool (89%), with overlaps between religious (36%), medicinal (50%) and ornamental (62%) plants; only 11% of species were weeds. Urban matrix parameters (age of development, housing density) and size and type of sacred sites were related to differences in species assemblages. We identified key species for different classes of age and housing density, and for types of sacred sites. Our study demonstrates that urban sacred sites have an important potential in harbouring both native and culturally significant species that can support urban livelihoods in developing countries by a range of cultural and provisioning ecosystem services, including medicinal uses. As such sites are conserved by communities for spiritual or cultural beliefs, local biodiversity can be enhanced, e.g. by adapting management practices through community participation. This would strengthen the important contribution of sacred sites within the green infrastructure of rapidly growing megacities.     

马铃薯AP2/ERF转录因子的克隆及植物表达载体构建

AP2/ERF基因家族转录因子普遍存在于植物中,参与植物体内的各种生物学过程,包括植物的生长发育、生物和非生物胁迫响应等。前期转录组测序的结果发现马铃薯‘加湘1号’一个AP2/ERF家族基因(PGSC0003DMG400012154)在接种晚疫病菌(Phytophthora infestans)24 h后被显著激活。从接种P.infestans 24 h的‘加湘1号’的总RNA中通过RT-PCR获得了该基因CDS序列为885 bp,BLAST分析显示该基因编码一个295个氨基酸残基的蛋白,并且含有1个AP2/ERF结构域,是AP2/ERF转录因子家族中的ERF亚家族的一员。本研究将该基因与XcmⅠ酶切的表达载体pCXSN连接,转化大肠杆菌,通过测序挑选插入正确克隆酶切验证,并成功转化农杆菌。本研究结果为进一步研究该基因的功能提供了帮助。     

Influencing in vitro clonal propagation of Chonemorpha fragrans(moon) Alston by culture media strength,plant growth regulators,carbon source and photo periodic incubation

Chonemorpha fragrans is an endangered medicinal woody climber,regarded among alternative plant sources of camptothecin.Camptothecin is a monoterpene indole anti-cancer alkaloid with annual trade value of over three billion U.S.dollars in the recent,and is used in the production of its analog drugs approved for the chemotherapy of cancer of varied types.Effects of plant growth regulators,culture media strength and photoperi-odic duration on the micropropagation ef ciency of C.fragrans from nodal segment explants were studied on Murashige and Skoog(MS)medium amended with Thidiazuron(TDZ),Benzylaminopurine(BAP)or Kinetin(Kin).Thidiazuron was more ef cient over BAP and Kin when half basal MS medium was used over full or quarter strength.Results of carbon source experiment showed sucrose as the most effective over glucose,fructose,and maltose in the clonal production.Studies on the photope-riodic incubation duration showed 12 h as the best light period and sub or supra-optimal resulted in the production of abnormal and albino micro shoots.Experimental results on the evaluation of physiological,biochemical parameters showed the role of pigment molecules and antioxidant systems in the production of albino micro shoots.     

栀子和玉兰花提取物对烤烟主要化学成分及致香物质含量影响

以湘烟5号为材料,通过盆栽试验,探讨不同浓度植物提取物对烤烟主要化学成分及致香物质含量的影响。结果表明,喷施栀子花和玉兰花汁液提取物有利于烟叶原料主要化学成分含量的改善,与对照相比,喷施处理的上部橘黄二级（B2F）和中部橘黄三级（C3F）烟叶还原糖含量分别高15.0%~21.1%和14.3%~17.7%,钾含量分别高5.7%~13.9%和7.4%~14.0%;与对照相比,喷施处理的烟叶的石油醚提取物、类胡萝卜素降解物、降解产物、茄酮、非酶棕色化反应产物、芳香族氨基酸裂解产物、新植二烯和中性致香物质总量,上部橘黄二级（B2F）烟叶分别高10.0%~20.9%、11.2%~20.9%、9.6%~20.2%、9.8%~21.1%、10.8%~22.5%、11.2%~23.0%、14.9%~24.7%和11.9%~22.1%,中部橘黄三级（C3F）烟叶分别高13.5%~24.1%、11.3%~21.0%、11.4%~20.0%、10.8%~18.7%、13.1%~21.2%、10.1%~19.1%、17.5%~27.0%和18.1%~27.6%;烟叶感官质量评价权重得分高于对照。栀子花和玉兰花汁液提取物对烟叶主要化学成分及致香物质含量的作用效果以施用量3.5~5.0g/株的处理最佳。     

A genetic linkage map in southern‐by‐spring oat identifies multiple quantitative trait loci for adaptation and rust resistance

We developed 178 recombinant inbred lines from a southern‐by‐spring oat population designated as “TxH.” These lines were genotyped to generate a high‐quality linkage map that resolved 6,902 markers into 21 linkage groups that matched closely with the latest hexaploid oat consensus map. Three major quantitative trait loci (QTLs) affecting heading date were found in locations that are consistent with known QTLs and candidate genes, and two other QTLs affecting heading date were found in novel locations. Five QTLs affecting plant height were found. Both sets of QTLs are responsible for transgressive segregation observed for these two traits. Four QTLs affecting resistance to crown rust, caused by the pathogen Puccinia coronata f. sp. avenae, were identified. Two of these QTLs are consistent with known clusters of rust resistance genes, while two may represent new locations of novel rust resistance genes. A complete set of SNP sequences suitable for generating markers for molecular selection is provided.     

河套地区种植方式与密度对'张杂谷19号'产量的影响

为明确河套地区不同栽培模式对'张杂谷19号'产量的影响,以新抗旱谷子杂交种'张杂谷19号'为试验材料,研究3种种植方式（干旱无膜、干旱覆膜及灌溉无膜）和5种密度（4.5万、9万、13.5万、22.5万、45万株/hm²）对'张杂谷19号'稳产和丰产性的影响。结果表明,正常灌溉条件下,'张杂谷9号'的产量随着种植密度的增加而增加。当密度为45万株/hm²时产量最高,达到8724.36 kg/hm²。而干旱条件下,适宜种植密度可以同样获得较高产量,与正常灌溉条件下最高产量差异不显著。当种植密度为9万~ 45万株/hm²时,产量普遍提高到7700 kg/hm²以上,最高可达8654.33 kg/hm²。河套地区'张杂谷19号'播前灌溉1次足水、地膜覆盖及种植密度9万~45万株/hm²,可以实现水分高效利用和产量达到较高水平。     

植物来源药物联合抗肿瘤的研究进展

主要对长春碱、小檗碱、喜树碱和苦参碱等生物碱类、紫杉醇类药物联合抗肿瘤治疗进行了综述,并指出植物来源药物联合抗肿瘤的发展方向。     

筒式结构拆除的力学分析

根据力学原理，分析讨论了钢筋混凝土筒式结构定向倾倒的切口高度和切口角度．     

人工培植羊体羊黄的研究

本试验以外科手术方法在北京农户所养绵羊胆囊内放人异物网核和注入致黄菌(SEBF8,50亿/ml),诱发羊黄,均获成功.其间,对术前术后血清胆红质、胆固醇、谷草转氨酶和GROS反应等项进行测定.结果表明:上述各项指标在术后8天内与术前比,均有降低,差异显著,但以后逐渐恢复正常.而体重、临床表现,以及饲养指标本出现异常.预示着,人工培植羊体羊黄可行.同时,本试验还为进一步提高人工培植羊黄的产黄量,提供了参考数据.